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Molecular studies on intraspecific diversity and phylogenetic position of Coniothyrium minitans

Muthumeenakshi, S.; Goldstein, A. L.; Stewart, Alison; Whipps, J. M. (Cambridge University Press, 2001-09)

Simple sequence repeat (SSR)–PCR amplification using a microsatellite primer (GACA)₄ and ribosomal RNA gene sequencing were used to examine the intraspecific diversity in the mycoparasite Coniothyrium minitans based on 48 ...

Examination of Trichoderma phylogenies derived from ribosomal DNA sequence data

Dodd, Sarah; Crowhurst, R. N.; Rodrigo, A. G.; Samuel, G. J.; Hill, Robert A.; Stewart, Alison (Cambridge University Press, 2000-01)

Ribosomal DNA sequences were assessed for their usefulness in distinguishing among Trichoderma isolates and for their robustness in resolving their phylogenetic relationships. DNA sequences from the D2 region of the 28S ...

Use of Coniothyrium minitans transformed with the hygromycin B resistance gene to study survival and infection of Sclerotinia sclerotiorum sclerotia in soil

Jones, Elizabeth E.; Stewart, Alison; Whipps, J. (Cambridge University Press, 2003-03)

A Coniothyrium minitans strain (T3) co-transformed with the genes for β-glucuronidase (uidA) and hygromycin phosphotransferase (hph), the latter providing resistance to the antibiotic hygromycin B, was used to investigate ...

Co-transformation of the sclerotial mycoparasite Coniothyrium minitans with hygromycin B resistance and [beta]-glucuronidase markers

Jones, Elizabeth E.; Carpenter, Margaret A.; Fong, D.; Goldstein, A. L.; Thrush, A.; Crowhurst, R. N.; Stewart, Alison (Cambridge University Press, 1999-08)

Coniothyrium minitans was successfully co-transformed with the uidA (β-glucuronidase) and the hygromycin-resistance (hph) genes. Both were under the control of the glyceraldehyde-3-phosphate promoter from Aspergillus ...

Development of an isolate-specific marker for tracking Phaeomoniella chlamydospora infection in grapevines

Ridgway, Hayley J.; Steyaert, Johanna M.; Pottinger, Brenda M.; Carpenter, Margaret A.; Nicol, David; Stewart, Alison (Mycological Society of America, 2005)

Petri disease causes decline of grapevines worldwide. The grapevine endophyte Phaeomoniella chlamydospora is the most important fungal pathogen associated with this disease. Epidemiological studies of this pathogen have ...

Co-expression of two genes, a chitinase (chit42) and proteinase (prb1), implicated in mycoparasitism by Trichoderma hamatum

Steyaert, Johanna M.; Stewart, Alison; Jaspers, Marlene V.; Carpenter, Margaret A.; Ridgway, Hayley J. (Mycological Society of America, 2004)

Mycoparasitism of fungal plant pathogens by Trichoderma species is a complex process that involves the production and coordinated secretion of cell-wall degrading enzymes. Genes implicated in mycoparasitism by Trichoderma ...