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Molecular studies on intraspecific diversity and phylogenetic position of Coniothyrium minitans
(Cambridge University Press, 2001-09)
Simple sequence repeat (SSR)–PCR amplification using a microsatellite primer (GACA)₄ and ribosomal RNA gene sequencing were used to examine the intraspecific diversity in the mycoparasite Coniothyrium minitans based on 48 ...
Protoplast isolation from shoots of asparagus cultures
(The University of Chicago Press, 1997-09)
Experiments to maximize the isolation and purification of viable protoplasts from shoot cultures of asparagus (Asparagus officinalis L.) were conducted. Important factors for high yield of viable protoplasts included: the ...
Culture and regeneration of protoplasts from shoots of asparagus cultures
(The University of Chicago Press, 1997-09)
A reliable culture and regeneration protocol has been established for asparagus (Asparagus officinalis L.) protoplasts isolated from shoots of in vitro plants. Studies focused on factors to maximize plating efficiency, ...
Examination of Trichoderma phylogenies derived from ribosomal DNA sequence data
(Cambridge University Press, 2000-01)
Ribosomal DNA sequences were assessed for their usefulness in distinguishing among Trichoderma isolates and for their robustness in resolving their phylogenetic relationships. DNA sequences from the D2 region of the 28S ...
Use of Coniothyrium minitans transformed with the hygromycin B resistance gene to study survival and infection of Sclerotinia sclerotiorum sclerotia in soil
(Cambridge University Press, 2003-03)
A Coniothyrium minitans strain (T3) co-transformed with the genes for β-glucuronidase (uidA) and hygromycin phosphotransferase (hph), the latter providing resistance to the antibiotic hygromycin B, was used to investigate ...
Temporal changes in height and diameter growth for two Nothofagus species in New Zealand
(Opulus Press, 1997-06)
We examined whether the growth dynamics of two species can explain their coexistence. In particular, we examined New Zealand forests dominated by Nothofagus fusca and N. menziesii to determine whether both species can reach ...
Co-transformation of the sclerotial mycoparasite Coniothyrium minitans with hygromycin B resistance and [beta]-glucuronidase markers
(Cambridge University Press, 1999-08)
Coniothyrium minitans was successfully co-transformed with the uidA (β-glucuronidase) and the hygromycin-resistance (hph) genes. Both were under the control of the glyceraldehyde-3-phosphate promoter from Aspergillus ...
Earthquake impacts in old-growth Nothofagus forests in New Zealand
(Opulus Press, 2001-06)
Six stands located on different land forms in mixed old-growth Nothofagus forests in the Matiri Valley (northwest of South Island, New Zealand) were sampled to examine the effects of two recent large earthquakes on tree ...
Tree species richness and turnover throughout New Zealand forests
(Opulus Press, 1999-12)
Patterns of mortality, recruitment, and forest turnover were investigated using permanent plot data from temperate forests in 14 localities throughout New Zealand. Tree mortality and recruitment rates were calculated from ...
Development of an isolate-specific marker for tracking Phaeomoniella chlamydospora infection in grapevines
(Mycological Society of America, 2005)
Petri disease causes decline of grapevines worldwide. The grapevine endophyte Phaeomoniella chlamydospora is the most important fungal pathogen associated with this disease. Epidemiological studies of this pathogen have ...