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dc.contributor.authorRidgway, Hayley J.en
dc.contributor.authorSteyaert, Johanna M.en
dc.contributor.authorPottinger, Brenda M.en
dc.contributor.authorCarpenter, Margaret A.en
dc.contributor.authorNicol, Daviden
dc.contributor.authorStewart, Alisonen
dc.date.accessioned2010-04-26T03:27:10Z
dc.date.issued2005en
dc.identifier.citationRidgway, H. J., Steyaert, J. M., Pottinger, B. M., Carpenter, M., Nicol, D., & Stewart, A. (2005). Development of an isolate-specific marker for tracking Phaeomoniella chlamydospora infection in grapevines. Mycologia, 97(5), 1093-1101.en
dc.identifier.issn0027-5514en
dc.identifier.urihttps://hdl.handle.net/10182/1748
dc.description.abstractPetri disease causes decline of grapevines worldwide. The grapevine endophyte Phaeomoniella chlamydospora is the most important fungal pathogen associated with this disease. Epidemiological studies of this pathogen have been hampered by its common occurrence in the internal tissue of apparently healthy vines. Development of a molecular marker for a single strain would overcome this limitation and aid experiments designed to answer key questions about the biology of this pathogen. Genetic variation analysis of New Zealand and Italian strains of P. chlamydospora detected a potential molecular marker in New Zealand isolate A21. Characterization of the 1010 bp marker band showed that it had 50% identity to moxY, a gene involved in the aflatoxin biosynthetic pathway of Aspergillus parasiticus. Sequencing of the region flanking the 1010 bp product revealed a single nucleotide polymorphism in the 3' border of the marker band. Primers were designed to amplify a 488 bp fragment encompassing this polymorphic site and cleavage of this product with the restriction enzyme BsrI produced three bands only in isolate A21 and two bands in all other isolates tested. The sensitivity of the PCR-RFLP protocol was increased with a nested PCR approach and the protocol optimized for soil and wood samples. When the nested PCR/RFLP procedure was used to determine the persistence of viable and nonviable spores in soil, the results showed that nonviable spores were undetected after 8 wk whereas viable spores still could be detected at 17 wk.en
dc.format.extent1093-1101en
dc.language.isoenen
dc.publisherMycological Society of Americaen
dc.relationThe original publication is available from - Mycological Society of America - http://www.mycologia.org/cgi/reprint/97/5/1093en
dc.rightsCopyright © 2005 by The Mycological Society of Americaen
dc.subjectmolecular markersen
dc.subjectPetri diseaseen
dc.subjectVitis viniferaen
dc.subjectPhaeomoniella chlamydosporaen
dc.subjectmonooxygenaseen
dc.subjectgrapevinesen
dc.subjectMycology & Parasitologyen
dc.subject.meshSporesen
dc.subject.meshAscomycotaen
dc.subject.meshVitisen
dc.subject.meshDeoxyribonucleases, Type II Site-Specificen
dc.subject.meshOxygenasesen
dc.subject.meshFungal Proteinsen
dc.subject.meshDNA, Fungalen
dc.subject.meshGenetic Markersen
dc.subject.meshElectrophoresis, Agar Gelen
dc.subject.meshSensitivity and Specificityen
dc.subject.meshPolymerase Chain Reactionen
dc.subject.meshSequence Analysis, DNAen
dc.subject.meshSoil Microbiologyen
dc.subject.meshPlant Diseasesen
dc.subject.meshAmino Acid Sequenceen
dc.subject.meshSequence Homology, Amino Aciden
dc.subject.meshPolymorphism, Geneticen
dc.subject.meshPolymorphism, Restriction Fragment Lengthen
dc.subject.meshPolymorphism, Single Nucleotideen
dc.subject.meshMolecular Sequence Dataen
dc.titleDevelopment of an isolate-specific marker for tracking Phaeomoniella chlamydospora infection in grapevinesen
dc.typeJournal Article
dc.subject.marsdenFields of Research::270000 Biological Sciences::270300 Microbiology::270305 Mycologyen
dc.subject.marsdenFields of Research::300000 Agricultural, Veterinary and Environmental Sciences::300300 Horticulture::300303 Plant protection (pests, diseases and weeds)en
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Pest Management and Conservationen
lu.contributor.unitBio-Protection and Ecologyen
lu.contributor.unitBio-Protection Research Centreen
lu.contributor.unitLincoln Agritechen
lu.contributor.uniten
lu.contributor.uniten
lu.contributor.unitSoil, Plants and Ecological Sciencesen
lu.contributor.unit/LU/SPES/PLANTen
dc.subject.anzsrc0605 Microbiologyen
dc.subject.anzsrc0607 Plant Biologyen
dc.relation.isPartOfMycologiaen
pubs.issue5en
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/ECOL
pubs.organisational-group/LU/BPEC
pubs.organisational-group/LU/BPRC
pubs.organisational-group/LU/Lincoln Agritech
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/2018 PBRF Staff group
pubs.organisational-group/LU/SPES
pubs.organisational-group/LU/SPES/PLANT
pubs.publication-statusPublisheden
pubs.publisher-urlhttp://www.mycologia.org/cgi/reprint/97/5/1093en
pubs.volume97en
lu.identifier.orcid0000-0002-2125-7257


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