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dc.contributor.authorDaniel, Philip Bruce
dc.date.accessioned2010-05-04T23:52:18Z
dc.date.available2010-05-04T23:52:18Z
dc.date.issued1994
dc.identifier.urihttps://hdl.handle.net/10182/1799
dc.description.abstractEukaryotic nuclear genes are regulated by a complex array of transcription factors that influence RNA polymerase II activity. Included among these factors are the cellular receptors for steroid hormones. The rabbit uteroglobin gene expression is positively stimulated by progesterone, and binding sites for the progesterone receptor have been identified in distal regions of the promoter. In a recent analysis of the uteroglobin promoter (Rider and Bullock (1988)), a proximal promoter binding activity was identified from nuclear extracts of progesterone-stimulated uterine endometrial tissue. Also discovered was an activity capable of suppressing the DNA binding activity. In this work, the repressor activity was purified from nuclear extracts. The cause of this activity was found to be fragments of genomic DNA. The promoter binding activity (UGPB) was then further characterised. UGPB-UG200 binding in gel-shift assays was found to be competed out by sheared genomic DNA. Analysis of the UG200 sequence and competition gel-shift assays with poly(dAdT) suggest that UGPB has some affinity for AT-rich stretches of DNA. There is some evidence that UGPB is induced by both progesterone and, to a lesser extent, oestrogen, but there is still no evidence that it directly affects uteroglobin expression. Many aspects of this DNA binding protein are unlike those of known transcription factors.en
dc.language.isoenen
dc.publisherLincoln Universityen
dc.rights.urihttps://researcharchive.lincoln.ac.nz/page/rights
dc.subjectuteroglobinen
dc.subjectgene expressionen
dc.subjectprogesteroneen
dc.subjecttranscriptionen
dc.subjectRNA polymerase IIen
dc.subjectoestrogenen
dc.subjecteukaryotic transcriptionen
dc.subjectgel filtration chromatographyen
dc.subjectuteroglobin promoteren
dc.titleControl of uteroglobin gene expressionen
dc.typeThesisen
thesis.degree.grantorLincoln Universityen
thesis.degree.levelDoctoralen
thesis.degree.nameDoctor of Philosophyen
dc.subject.marsdenFields of Research::250000 Chemical Sciences::250300 Organic Chemistry::250302 Biological and medical chemistryen
dc.subject.marsdenFields of Research::270000 Biological Sciences::270100 Biochemistry and Cell Biologyen
lu.thesis.supervisorBullock, D.W.
lu.thesis.supervisorHickford, J.
lu.thesis.supervisorMolloy, C.
lu.contributor.unitDepartment of Wine, Food and Molecular Biosciencesen
dc.rights.accessRightsDigital thesis can be viewed by current staff and students of Lincoln University only. Print copy available for reading in Lincoln University Library. May be available through inter-library loan.en


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