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dc.contributor.authorJones, Elizabeth E.en
dc.contributor.authorStewart, Alisonen
dc.contributor.authorWhipps, J.en
dc.date.accessioned2008-03-25T20:43:39Z
dc.date.issued2003-03en
dc.identifier.citationJones, E. E., Stewart, A., & Whipps, J. M. (2003). Use of Coniothyrium minitans transformed with the hygromycin B resistance gene to study survival and infection of Sclerotinia sclerotiorum sclerotia in soil. Mycological Research, 107(3), 267-276.en
dc.identifier.issn0953-7562en
dc.identifier.urihttps://hdl.handle.net/10182/373
dc.description.abstractA Coniothyrium minitans strain (T3) co-transformed with the genes for β-glucuronidase (uidA) and hygromycin phosphotransferase (hph), the latter providing resistance to the antibiotic hygromycin B, was used to investigate the survival and infection of sclerotia of Sclerotinia sclerotiorum by C. minitans over time in four different soils. Infection of sclerotia was rapid in all cases, with the behaviour of transformant T3 and wild type parent A69 being similar. Differences were seen between the soils in the rate of infection of sclerotia by C. minitans and in their indigenous fungal populations. Amendment of agar with hygromycin B enabled the quantification of C. minitans in soil by dilution plating where there was a high background of other microorganisms. In Lincoln soil from New Zealand, which had a natural but low population of C. minitans, the hygromycin B resistance marker allowed the umambiguous discrimination of the applied transformed isolate from the indigenous hygromycin B sensitive one. In this soil, although the indigenous C. minitans population was detected from sclerotia, none were recovered on the dilution plates, indicating the increased sensitivity of C. minitans detection from soil using sclerotial baiting. C. minitans was a very efficient parasite, being able to infect a large proportion of sclerotia within a relatively short time from an initially low soil population. The addition of hygromycin B to agar also allowed the detection of C. minitans from decaying sclerotia by inhibiting secondary fungal colonisers. This is the first report to show that fungi colonising sclerotia already infected by C. minitans mask the detection of C. minitans from sclerotia rather than displacing the original parasite.en
dc.format.extent267-276en
dc.language.isoenen
dc.publisherCambridge University Pressen
dc.relationThe original publication is available from - Cambridge University Press - https://doi.org/10.1017/S0953756203007457en
dc.relation.urihttps://doi.org/10.1017/S0953756203007457en
dc.rightsCopyright © The British Mycological Society 2003en
dc.subjectsclerotiaen
dc.subjectinfectionen
dc.subjectConiothyrium minitansen
dc.subjectSclerotinia sclerotiorumen
dc.subjectsoil fungien
dc.subjectsoil mycologyen
dc.subjectMycology & Parasitologyen
dc.subject.meshAscomycotaen
dc.subject.meshGlucuronidaseen
dc.subject.meshPhosphotransferases (Alcohol Group Acceptor)en
dc.subject.meshHygromycin Ben
dc.subject.meshCulture Mediaen
dc.subject.meshColony Count, Microbialen
dc.subject.meshSoil Microbiologyen
dc.subject.meshDrug Resistance, Fungalen
dc.subject.meshTransformation, Geneticen
dc.titleUse of Coniothyrium minitans transformed with the hygromycin B resistance gene to study survival and infection of Sclerotinia sclerotiorum sclerotia in soilen
dc.typeJournal Article
dc.subject.marsdenFields of Research::270000 Biological Sciences::270300 Microbiology::270305 Mycologyen
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Pest Management and Conservationen
lu.contributor.unitBio-Protection Research Centreen
lu.contributor.uniten
lu.contributor.uniten
dc.identifier.doi10.1017/S0953756203007457en
dc.subject.anzsrc0605 Microbiologyen
dc.subject.anzsrc0607 Plant Biologyen
dc.relation.isPartOfMycological Researchen
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/ECOL
pubs.organisational-group/LU/BPRC
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/2018 PBRF Staff group
pubs.publication-statusPublisheden
pubs.volume107en
lu.identifier.orcid0000-0002-1879-4537


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