Variation in the Yak calpastatin gene (CAST)
Calpastatin (CAST) is a specific inhibitor of calpain (calcium-dependent cysteine protease). This study investigated the potential for variation in yak (Bos grennies) CAST. PCR-SSCP analysis of exon 6 of yak CAST revealed three unique patterns (named A-C). Sequencing of the amplicons revealed two nucleotide substitutions. One substitution (c.398G/C) would nominally change the amino acid sequence (p.S133T) of yak calpastatin. The variant sequence A which carried c.398C was the most common in the yaks tested (95.1%). This is the first report that found yak CAST is variable, and as in pigs, sheep and cattle, this variation may affect animal production traits.... [Show full abstract]
KeywordsCAST; genetic variation; Yak (Bos grennies); PCR- single strand conformational polymorphism (PCR-SSCP)
Copyright©2012 Yang G., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
CitationYang G., Zhou H., Hu J., Luo Y. & Hickford, J.G.H. (2012). Variation in the yak calpastatin gene (CAST). International Journal of Genetics, 4(1) pp.-72-73.
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