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dc.contributor.authorChai, Wenqiongen
dc.contributor.authorZhou, Huitongen
dc.contributor.authorForrest, Rachelen
dc.contributor.authorGong, Huaen
dc.contributor.authorHodge, Simonen
dc.contributor.authorHickford, Jonathan G. H.en
dc.date.accessioned2017-11-22T20:26:19Z
dc.date.available2017-08-31en
dc.date.issued2017-10-01en
dc.date.submitted2017-08-28en
dc.identifier.citationChai, Wenqiong, Zhou, Huitong, Forrest, Rachel H.J., Gong, Hua, Hodge, Simon, Hickford, Jon G.H., Polymorphism of KRT83 and its association with selected wool traits in Merino-cross lambs.Small Ruminant Research http://dx.doi.org/10.1016/j.smallrumres.2017.08.019en
dc.identifier.issn0921-4488en
dc.identifier.urihttps://hdl.handle.net/10182/8779
dc.description.abstractKeratins are structural proteins in the cortex of wool fibres. It is thought that variation in the keratin genes (KRTs) affects wool structure and characteristics. Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) analysis was used to investigate three regions of ovine KRT83. These regions were a portion of the promoter, the exon 2 region (including part of intron 1) and a region encompassing exon 3-4 (including all of exon 3, intron 3, exon 4 and part of intron 4). Initially, in 300 New Zealand Romney, Merino and White Dorper sheep obtained from 26 farms, one, two and four PCR-SSCP banding patterns were observed for these regions respectively. The exon 2 region contained two single nucleotide polymorphisms (SNPs) and the exon 3-4 region contained five SNPs. Investigation of the effect of the variation in the exon 3-4 region on variation in some wool traits were subsequently undertaken in 489 Merino × Southdown-cross sheep from seven sire-lines. The four variants identified in the original 300 sheep (designated A-D) and a new variant (E containing a new SNP) were observed with a frequency of 64.6%, 15.4%, 6.6%, 10.1% and 3.3%, respectively. General linear mixed-effects models (GLMMs) were used to investigate associations between the presence or absence of the variants and the wool traits, with a second set of models testing associations between common genotypes and those traits. The presence of A was associated with a decrease in fibre diameter standard deviation (FDSD) and coefficient of variation of fibre diameter (CVFD). The presence of C was associated with an increase in mean fibre diameter (MFD), mean fibre curvature (MFC) and prickle factor (PF), and a decrease in wool yield (Yield) [clean fleece weight (CFW)/greasy fleece weight (GFW) × 100%]. A trend for association between the presence of C and increased FDSD was also detected. The presence of D was associated with an increase in MFD and PF, and a decrease in Yield. The presence of E was associated with a decrease in CVFD. Genotype AD had a higher GFW and a lower Yield than AA, and AC and AD tended to have increased MFD compared to AA and AB. These results suggest that ovine KRT83 might be a useful candidate gene for improving wool traits.en
dc.format.extent6-11en
dc.language.isoenen
dc.publisherElsevieren
dc.relationThe original publication is available from - Elsevier - https://doi.org/10.1016/j.smallrumres.2017.08.019en
dc.relation.urihttps://doi.org/10.1016/j.smallrumres.2017.08.019en
dc.rights© 2017 Elsevier B.V. All rights reserved.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectKRT83en
dc.subjectvariationen
dc.subjectwool traitsen
dc.subjectsheepen
dc.subjectDairy & Animal Scienceen
dc.titlePolymorphism of KRT83 and its association with selected wool traits in Merino-cross lambsen
dc.typeJournal Article
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Agricultural Sciencesen
dc.identifier.doi10.1016/j.smallrumres.2017.08.019en
dc.subject.anzsrc070201 Animal Breedingen
dc.subject.anzsrc070299 Animal Production not elsewhere classifieden
dc.subject.anzsrc0702 Animal Productionen
dc.relation.isPartOfSmall Ruminant Researchen
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/AGSC
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/QE18
pubs.publication-statusPublisheden
pubs.volume155en
dc.rights.licenceAttribution-NonCommercialen
lu.identifier.orcid0000-0001-7620-3160
lu.identifier.orcid0000-0002-2247-3824
lu.identifier.orcid0000-0001-9313-761X
lu.identifier.orcid0000-0001-6933-5253


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